Dr. James Dunn
Dr. James Dunn has a background in biology, engineering, and medicine. Dr. Dunn obtained his B.S. degree in Biology and Chemical Engineering from the California Institute of Technology and his M.D. and Ph.D. degrees from Harvard and Massachusetts Institute of Technology. His research interest focuses on tissue engineering and regenerative medicine of the gastrointestinal system. Dr. Dunn’s laboratory is part of the NIH Intestinal Stem Cell Consortium, and is also funded by the California Institute for Regenerative Medicine for neural progenitor cell therapy. Dr Dunn has extensive experience with animal models of intestinal disease. His lab developed an in vivo model to test the ability of intestinal stem cells to self-renew and to differentiate into various cell lineages. Dr. Dunn is also developing a device that will induce growth and lengthening of the intestine.
To generate missing tissue for infants and children with organ insufficiency
- Understand the interaction between stem cells and their environment
- Develop cell-based therapy for pediatric disorders
- Define the effect of mechanical force on cell and tissue growth
After pursuing his graduate training in Maryland, Chih-hsin moved west to enjoy the sunshine in California.
He joined Stanford and began to explore various molecular pathways and animal models in order to understand the fundamentals of cell development and its relationship to disease progression. In addition to routine bench work, Chihhsin feels honored to have the opportunity to cooperate with teams to set up and manage labs. Currently he is focusing on utilizing enteric stem cells to advance his studies.
Chihhsin also devotes himself to spending time with his family and building LEGO models while he is off duty.
Hadi Hosseini is a postdoctoral research fellow. He received his PhD in Biomedical Engineering and Biophysics from Washington University in 2017.
He has extensive experience in using Multiphysics (FEA/FEM) modeling for biomechanics and bio-field applications, calibrating models, and optimizing input parameters using in vitro data. In the lab, Hadi is working on the short bowel syndrome (SBS) project, dedicated to helping patients born with SBS. Recent experimental observations have seen that mechanical distraction may play a role in a potential cure, although some technical complications should be addressed. Hadi is trying to use his finite element modeling, programming and my scientific knowledge to investigate the role of mechanical forces as a tool for intestinal lengthening.
Hadi enjoys playing sand volleyball, spike ball, biking, and exploring the lovely Bay area!
Po-Yu (Steve) Lin
Steve Lin is a research associate in Dr. Dunn's Lab. In 2013, he received his Master's Degree in Bioengineering at UCLA and is currently working to complete his PhD as well.
Steve's research interests include stem cell therapy and tissue engineering. The focus of his research is regarding intestinal stem cell niche and its differentiation. For his project, Steve is studying the effects of biochemical and environmental cue on the differentiation of stem cells into mature intestinal epithelium. He has developed a culturing model that allows long term culture of intestinal epithelium in 2D which provides a better platform for the study of epithelium in vitro. Steve is also studying the interaction between differentiated epithelium and intestinal subepithelial myofibroblasts.
Other than doing research, Steve enjoys reading, watching movies, playing tennis, getting kicked and punched in Muay Thai class, and working out with loud music in the background. He is an avid coffee drinker, and can be found either in the coffee shop or on his way there.
Steve can be reached at firstname.lastname@example.org
Tommy is a temporary research employee in Dr. James Dunn’s lab.
In 2018, he completed his B.S. in Bioengineering with an emphasis on biomaterials and regenerative medicine at UCLA. Prior to Dr. Dunn’s arrival at Stanford from UCLA in 2016, Tommy was an undergraduate research assistant in his lab. He later rejoined the lab in 2018 at Stanford while he takes a gap year to apply for an M.S. in Bioengineering. Currently, his research focuses on designing and fabricating three-dimensionally printed polymeric springs for distraction enterogenesis to treat short bowel syndrome.
In his free time, Tommy likes hiking, traveling, cooking, eating brunch, and watching YouTube.
Katherine "KP" Portelli
Katherine (KP) Portelli is a MD candidate and Research Fellow in Dr. James Dunn’s lab at Stanford.
KP received her B.A. in Film Production at Villanova University and is currently a rising MS4 at Zucker SOM at Hofstra/Northwell in Hempstead, NY.
KP focuses on the utility of distraction enterogenesis in murine large intestine. She is studying spring-mediated distraction enterogenesis in the murine cecum and resultant adaptive changes.
KP is also studying the architecture of the colonic muscularis propria. Specifically, she evaluates the maturation and growth of the teniae coli in both porcine and human populations.
In addition, KP developed a database to analyze clinical outcomes associated with surgical and non-surgical management of pediatric short bowel syndrome.
Jordan Taylor is a postdoctoral research fellow in the Division of Pediatric Surgery.
Jordan is a graduate of UC San Diego School of Medicine and is a current general surgery resident in New York with an interest in pediatric surgery. His research in the lab is focused on the use of skin-derived precursor cells for the treatment of Hirschprung's Disease, and other diseases involving enteric nervous system dysfunction. Jordan's other project is investigating the use of stem cells to regenerate enteric epithelium in a murine model of enterocyte dysfunction causing iron deficiency anemia.
Outside the lab, Jordan enjoys the California sun, being close to family, and spending time with his new wife, Kristie.
Anne-Laure primarily joined Dr Dunn's lab in June 2015 as an Intern Student while completing her Masters Degree in Bioengineering in Nice, France.
After graduating in July 2016, she started her new position as the Lab Manager. Anne-Laure's research focuses on tissue engineering and regenerative medicine. Her first project is to derive multipotent skin cells to grow neuroglial precursors for the treatment of neuromuscular dysfunction. She is also designing and 3D-printing implantable surgical devices for intestinal lengthening.
In her free time, Anne-Laure likes to be close to the ocean, enjoys attending dance classes, and exploring San Francisco.
Lauren is a research resident in the lab of Dr. James Dunn.
In 2016, she received her MD from Stanford and stayed to pursue her residency training in General Surgery. Lauren joined Dr. Dunn's lab in 2018 during her professional development years. Her research is focused on developing methods to denude the epithelial lining of the small intestine to prime the tissue to engraft intestinal stem cells. Lauren is also analyzing the cellular and molecular mechanisms driving spring distraction enterogenesis, or how segments of bowel lengthen when exposed to stretch.
Outside of the lab and hospital, Lauren enjoys all things food (eating, cooking, baking), reading, travelling and spending time with friends and family.
David is a student intern in the lab of Dr. James Dunn.
David is an undergraduate student at UC Davis majoring in microbiology. His research interests focus on the effect that butyrate has upon culturing epithelial monolayers with intestinal stem cells in a collagen layer. He aims to determine whether butyrate influcense the differentiation and growth rate of these cells.
Outside of the lab, David enjoys going camping, backpacking, and visiting the beaches in California. He also likes to work out and care for his collection of succulent and cacti.
Abstracts and Presentations
Three-dimensionally printed surface features to anchor endoluminal spring for distraction enterogenesis
"Springs with bidirectional hooked surface features were anchored to the intestine for up to 4 weeks without migration. Bidirectional features printed on springs prevented the premature migration of endoluminal springs. These novel spring anchors allowed for their endoluminal placement without any sutures."
Spontaneous and Periodic Contractions of Murine and Human Intestinal Muscularis Cells
Mechanically induced development and maturation of human intestinal organoids in vivo
"... we show that the incorporation of uniaxial strain, using compressed nitinol springs, in human intestinal organoids transplanted into the mesentery of mice induces growth and maturation of the organoids."
Double Plication for Spring-Mediated in-Continuity Intestinal Lengthening in a Porcine Model
Dr. Dubrovsky presented research done with Dr. James Dunn at the 13th Annual Academic Surgical Congress
A Novel Culture System for Adult Porcine Intestinal Crypts
A new "method [was developed that maintains] juvenile and adult porcine crypt cells long-term in culture. Porcine enteroids and spheroids can be successfully passaged and transduced by using lentiviral vectors."
Intestinal Matrix Prevents Therapeutic Ultrasound from Causing Inertial Cavitation in Tumescent Subcutaneous Tissue
Dr. James Dunn collaborated with the University of California at Los Angeles's Physics Department to find that subcutaneous tisseu campens inertial cavitation.
Basic Fibroblast Growth Factor eluting Microspheres Enhance Distraction Enterogenesis
"Sustained release bFGF microspheres enhanced distraction enterogenesis through improved vascularity. The synergy of growth factors such as bFGF with distraction enterogenesis may yield improved results for the future treatment of patients with short bowel syndrome."